课程介绍

 

　　Learnaboutthetechnologiesunderlyingexperimentationusedinsystemsbiology,withparticularfocusonRNAsequencing,massspec-basedproteomics,flow/masscytometryandlive-cellimaging.Akeydriverofthesystemsbiologyfieldisthetechnologyallowingustodelvedeeperandwiderintohowcellsrespondtoexperimentalperturbations.Thisinturnsallowsustobuildmoredetailedquantitativemodelsofcellularfunction,whichcangiveimportantinsightintoapplicationsrangingfrombiotechnologytohumandisease.Thiscoursegivesabroadoverviewofavarietyofcurrentexperimentaltechniquesusedinmodernsystemsbiology,withfocusonobtainingthequantitativedataneededforcomputationalmodelingpurposesindownstreamanalyses.Wedivedeeplyintofourtechnologiesinparticular,mRNAsequencing,massspectrometry-basedproteomics,flow/masscytometry,andlive-cellimaging.Thesetechniquesareoftenusedinsystemsbiologyandrangefromgenome-widecoveragetosinglemoleculecoverage,millionsofcellstosinglecells,andsingletimepointstofrequentlysampledtimecourses.Wepresentnotonlythetheoreticalbackgrounduponwhichthesetechnologieswork,butalsoenterrealwetlabenvironmentstoprovideinstructiononhowthesetechniquesareperformedinpractice,andhowresultantdataareanalyzedforqualityandcontent.

 

　　课程目录

 

　　1Introduction

 

　　1.1Lecture1-ScopeandOverview

 

　　1.2Lecture2-BiologicalModelSystems

 

　　1.3Lecture3-ExperimentalPerturbations

 

　　1.4Lecture4-MeasuringNucleicAcids

 

　　1.5Lecture5-MeasuringProteinandProteinStates